Tuesday, October 22, 2013

More samples and Strep throat swabbing practice

We are now half way through our tests for our mystery bacteria H. Today we looked at the streak plate we did last week. The bacteria grew on the surface so it must be aerobic. To see if bacteria H has any peroxisomes we poured hydrogen peroxide on the surface of the plate. Our's was slow to bubble, but after a few seconds it started to react. This told us that our bacteria is definitely aerobic because the catalyst needs to be present in order for there to be oxygen for the bacteria to grow.

We shared our plate with another group so our bacteria is the yellow one.


 We also inoculated seven more tests. Five of them are called differential media tests, one was to test for the hydrolytic enzymes for DNA, and one was to test the anaerobic or aerobic type of bacteria.

Anaerobic or Aerobic?
After inoculating 
Anaerobic or Aerobic?
Before inoculating 



The five test we started on different media were:
EMB, Blood, PE, Mannitol Salt Agar, and MacConkey Agar





















We got to be all official and use the disposable tips to inoculate!!
So the last thing we did was practice swabbing each other's throats for strep. We didn't test them all, but we got to practice the technique. It was super fun. hardly anyone gagged so I hope we did it right! Anyway, on Thursday we will see if our bacteria has decided to be interesting or not. We shall see.

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